Journal: PLoS ONE

Article Title: Loss of the Orphan Nuclear Receptor SHP Is More Pronounced in Fibrolamellar Carcinoma than in Typical Hepatocellular Carcinoma

doi: 10.1371/journal.pone.0030944

Figure Lengend Snippet: A, B – immunohistochemical staining of the normal hepatic lobule; C, D- immunofluorescence staining showing exclusively nuclear (C) or cytoplasmic (D) SHP localization in different lobules; scale bar: A 100 µm; B 20 µm; C,D 25 µm. E- result of the SHP immunohistochemistry preceded with (lower image) or without (upper image) anti-SHP blocking antibody; F- Western blot analysis of three liver (LV1–LV3) lysates, the SHP protein present in a single band; G- Result of the RT-PCR study showing the SHP mRNA in the normal liver; GAPDH mRNA was used as a control.

Article Snippet: As a control immunoreaction, SHP antibody, pre-incubated with specific SHP peptide (MBL International, USA) with a 40× molar excess of the peptide prior to application into tissue was used.

Techniques: Immunohistochemical staining, Staining, Immunofluorescence, Immunohistochemistry, Blocking Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Scientific Reports

Article Title: Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening

doi: 10.1038/srep35544

Figure Lengend Snippet: ( a ) Immunofluorescence images of CK-14 (in red). ( b ) Immunofluorescence images of IL-6 (in green). ( c ) Quantified results of the immunofluorescence images by the standardized intensity; the error bars represent the standard deviations calculated from separate assays (n = 3), (*p < 0.05). Scale bar of all images: 100 μm.

Article Snippet: In order to further study the role of IL-6 expression in cancer cells migration, IL-6 inhibition experiments on MDA-MB-231 cells were performed by 24 h of pre-incubation using IL-6 receptor antibody (IL-6 RA, ab47215, Abcam).

Techniques: Immunofluorescence